Polymorphisms in the hypervariable control region of the mitochondrial DNA differentiate BPH populations

The brown planthopper (BPH; Nilaparvata lugens ) is one of India’s most destructive pests rice. BPH, a monophagous migratory insect, reported from all major rice-growing ecosystems the country, capable traversing large distances and causing massive crop loss. A crucial step for developing viable management strategies understanding its population dynamics. Very few reliable markers are currently available to screen BPH populations their diversity. In current investigation, we developed combinatorial approach using polymorphism present within mitochondrial Control Region in nuclear genome (genomic simple sequence repeats; gSSRs) unravel diversity collected various regions India. Using two specific primer pairs, complete (1112 2612 bp) was PCR amplified as overlapping fragments, cloned sequenced individuals representing nine different populations. Results revealed extensive this region due variable number tandem repeats. three selected gSSR also exhibited population-specific amplification patterns. Overall genetic between high (>5%). Further, silico double-digestion consensus sequences Region, with HpyCH4IV Tsp45I restriction enzymes, unique fragment length polymorphisms (digital-RFLPs; dRFLPs) that differentiated To best our knowledge, first report mitogenome can differentiate Eventually, such rapid marker-based identification will pave way an efficient pest strategy.